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Extra info for Animals in Scientific Research: An Effective Substitute for Man?: Proceedings of a Symposium held in April 1982 under the auspices of the Humane Research Trust

Example text

Likely stages in the development of an in vitro toxicity test The most appropriate use of in vitro tests is for the screening of chemicals for a particular adverse property such as enzyme inhibition and uncoupling of mitochondria. It follows that an effective , solely in vitro, approach to toxicity testing depends on the assumption that: i) the wide range of toxic signs and symptoms observed in vivo are triggered by a relatively small number of identifiable initiating events. (An outline of the mechanisms which may be involved in the development of a toxic lesion is presented in figure 2).

In Handbook of Mutagenicity Test Procedures. (ed . J. ) Elsevier Scientific Publishing Co. Amsterdam. 44 Styles, J. A. (1977). A method for detecting carcinogenic organic chemicals using mammalian cells in culture. Cancer 36, 558-563. Trosko, J . E. , Yotti, L. P. and Chu, E. H. Y. (1977). Effect of phorbol myristate acetate on the recovery of spontaneous and ultraviolet light induced 6-thioguanine and oubain resistant Chinese hamster cells. Cancer Res . 37 , 188-193 . 45 The design and use of in vitro toxicity tests James W.

UKEMS Collaborative genotoxicity trial, cytogenetic tests of 4CMB, BC and 4HMB: Summary and appraisal. Mutation Res. 100, 313-331. --Searle, A. G. (1977). The specific locus test in the mouse. In Handbook of Mutagenicity Test Procedures. (ed . J. ) Elsevier Scientific Publishing Co. Amsterdam. 44 Styles, J. A. (1977). A method for detecting carcinogenic organic chemicals using mammalian cells in culture. Cancer 36, 558-563. Trosko, J . E. , Yotti, L. P. and Chu, E. H. Y. (1977). Effect of phorbol myristate acetate on the recovery of spontaneous and ultraviolet light induced 6-thioguanine and oubain resistant Chinese hamster cells.

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