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By Davor Ježek

Atlas at the Human Testis: general Morphology and Pathology provides histological illustrative fabric from paraffin and semi-thin sections of the human testis that are usually utilized in the evaluate of testicular morphology, permitting an early detection of carcinoma in situ and extra complex pathological adjustments of the testicular parenchyma. The early detection of melanoma in situ is predicated at the cautious morphological research of the biopsy and immunohistochemistry (IHC). consequently, this atlas comprises exact descriptions of IHC tools in addition to smooth molecular organic equipment corresponding to DNA microarrays and proteomics and complex microscopy recommendations concerning the testicular biopsy.

Adequate evaluate of the testicular biopsy results in excessive healing premiums of testicular neoplasms which are used as a foundation to effectively deal with infertility in males.

Atlas at the Human Testis: common Morphology and Pathology is a worthwhile reference software with the intention to attract andrologists, urologists, pathologists, medical embryologists, in addition to reproductive biology scientists.

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Additional resources for Atlas on the human testis : normal morphology and pathology

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1999;72(4):666–9. Kanto S, Sugawara J, Masuda H, Sasano H, Arai Y, Kyono K. Fresh motile testicular sperm retrieved from nonobstructive azoospermic patients has the same potential to achieve fertilization and pregnancy via ICSI as sperm retrieved from obstructive azoospermic patients. Fertil Steril. e5–7. Levran D, Ginath S, Farhi J, Nahum H, Glezerman M, Weissman A. Timing of testicular sperm retrieval procedures and in vitro fertilization-intracytoplasmic sperm injection outcome. Fertil Steril.

Therefore, the bioptic material should be checked routinely for malignant changes. Tissue sections 3–5 mm thick are cut from paraffin-embedded tissue blocks, as described earlier, and placed on object silanecoated slides. The slides containing tissue sections are incubated for 20 min at 60 °C. 0) in a microwave oven at 800 and 400 W for antigen retrieval. 2), and the endogenous peroxidase activity (optional; some new kits do not require this step) is blocked by a 5-min treatment with H2O2. , placental alkaline phos- phatase) at room temperature.

Ramasamy R, Fisher ES, Ricci JA, Leung RA, Schlegel PN. Duration of microdissection testicular sperm extraction procedures: relationship to sperm retrieval success. J Urol. 2011a;185(4):1394–7. Ramasamy R, Reifsnyder JE, Bryson C, Zaninovic N, Liotta D, Cook CA, et al. Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction. Fertil Steril. 2011b;96(2):299–302. Schlegel PN. Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision.

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