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Viscosus: Immunoregulation 53 ly three discrepancies were discernible (leJeune and Burckhardt, unpublished data). , 1977). Furthermore, it was interesting to observe the PBA-property of strain N y l mitogens for human Β cells in culture and to follow their differentiation into Β blasts, immature plasmablasts, and finally plasma cells. In contrast to pokeweed mitogen, their induction was independent of the help of Tcells which had been removed from peripheral blood leukocytes drawn from healthy volunteers by prior rosetting with sheep erythrocytes (Burckhardt and Cooper, unpublished data).

Three weeks after the start of the weekly injections, the rats were associated orally with viable cultures of the N y l strain which rapidly forms extensive dental plaque. When the experiment was terminated six weeks later, blood was collected from the animals; their lower jaws were processed for standardized evaluation of the distance between the alveolar bone crest (ABC) and the cemento-enamel junction (CE J) and their upper jaws were pro­ cessed for histometric measurements on random semithin section.

A . James T. Powell Werner Fischlsch weiger Department of Basic Dental Sciences College of Dentistry University of Florida Gainesville, Florida, U. S. A . I. INTRODUCTION Recent investigations have strongly implicated certain members of the oral microbiota as possible etiologic agents in various forms of periodontal dis­ eases. Actinomyces viscosus, a Gram-positive, facultative member of the nor­ mal oral microbiota, has been implicated as a causative agent in gingivitis (Loesche and Syed, 1978; Syed and Loesche, 1978).

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