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By Paul B. Fisher

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Extra info for Cancer Genomics and Proteomics: Methods and Protocols

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Gov/SAGE) holds 277 human libraries, 143 mouse libraries, and a variety of libraries of other species (December 2004). This unique resource allows investigators to query the expression of a gene in a large sample of human tissues in cancer. The SAGE map website also hosts programs to analyze SAGE data. Digital Northerns can be applied to any gene for which a SAGE tag is known. In this way, the expression of a gene of interest can be determined in a large collection of tissues and cancers. In addition statistical comparisons of SAGE libraries are available through SAGEmap.

Et al. (2002) Identification and cloning of human astrocyte genes displaying elevated expression after infection with HIV-1 or Cloning Differentially Expressed Genes Using RaSH 29 exposure to HIV-1 envelope glycoprotein by rapid subtraction hybridization, RaSH. Oncogene 21, 3592–3602. 20. Corton, J. C. and Gustafsson, J. A. (1997) Increased efficiency in screening large numbers of cDNA fragments generated by differential display. Biotechniques 22, 802–804, 806, 808. 21. Jiang, H. and Fisher, P.

Developments in SAGE Technology The initial SAGE method, described by Velculescu et al. (1995), uses the type-2 restriction enzyme BsmFI to generate SAGE tags of approx 14 nucleotides (nt).

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