Download Cancer Vaccines: Methods and Protocols by Michael J.P. Lawman, Patricia D. Lawman PDF

By Michael J.P. Lawman, Patricia D. Lawman

Cancer Vaccines: tools and Protocols explores the manipulation and amendment of immune cells, the manipulation and amendment of tumor cells in addition to the manipulation of immune/tumor interactions and numerous supply mechanisms, with the final finish objective of evoking a tumor-specific reaction and overcoming the immuno-evasive mechanisms hired by means of the tumor cells. This designated quantity additionally covers the topic of melanoma vaccines in a extra international feel with its part at the advances, demanding situations, and way forward for melanoma vaccines. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, effortlessly reproducible laboratory protocols and pointers on troubleshooting and warding off recognized pitfalls.

Comprehensive and authoritative, Cancer Vaccines: tools and Protocols goals to aid consultant researchers towards constructing extra generations of melanoma vaccines which are either secure and efficacious, with the wish that melanoma vaccines may be the ordinary of care within the very close to future.

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3. 15-mL polypropylene tubes. 4. IMDM medium with 10 % FBS. 6 DC Harvest and Storage 1. Cryotubes. 2. 2× freezing medium A: RPMI-1640 and 40 % FBS. 3. 2× freezing medium B: RPMI-1640 and 20 % dimethyl sulfoxide (DMSO). 4. Cryo 1 °C Nalgene™, freezing container (Thermo Fisher Scientific Inc, Rochester, NY, USA) with isopropanol. 7 Phenotypic Analysis of Multiple Peptide Cocktail-Pulsed DC 1. Mouse antihuman CD80 mAb conjugated with PE (Becton Dickinson Pharmingen). 2. Mouse antihuman CD83 mAb conjugated with FITC (Becton Dickinson Pharmingen).

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Cancer Immunol Immunother 54:187–207 3. Chiang CL et al (2010) Whole tumor antigen vaccines. Semin Immunol 22:132–143 4. Ribas A et al (2010) Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma. J Transl Med 8:89 5. Prins RM et al (2011) Gene expression profile correlates with t-cell infiltration and relative survival in glioblastoma patients vaccinated with dendritic cell immunotherapy. Clin Cancer Res 17:1603–1615 6. Herzog GI et al (2011) Quality of tumor lysates used for pulsing dendritic cells is influenced by the method used to harvest adherent tumor cells.

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